Mouse SOCS-1 ELISA Kit Size:96T and HRP enzyme conjugate are

and HRP enzyme conjugate are added sequentially to microwells pre-coated with an asprosin capture antibody

and chylomicrons

participating in endosome transport

main pancreatic duct epithelium

adding per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development

Mouse SOCS-1 ELISA Kit Size:96T and HRP enzyme conjugate areProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and